Journal: STAR Protocols

Article Title: Protocol for real-time imaging, polar protein quantification, and targeted laser ablation of regenerating shoot progenitors in Arabidopsis

doi: 10.1016/j.xpro.2023.102184

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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Murashige & Skoog Basal Salts Medium Sigma Cat# M5524 Agar plant cell culture tested, powder Sigma Cat# A7921 MES hydrate Sigma Cat# M2933 Gamborg’s B5 basal salt mixture Sigma Cat# G5768 Gamborg’s vitamin solution 1000× Sigma Cat# G1019 Sucrose Sigma Cat# S0389 D-Glucose Sigma Cat# G7021 2,4-Dichlorophenoxyacetic acid (2,4-D) Sigma Cat# 49083 Kinetin Sigma Cat# K3378 Trans-zeatin Sigma Cat# Z0876 Biotin Sigma Cat# B4639 Propidium iodide (PI) Sigma Cat# P4170 Ampicillin Sigma Cat# A0166 Sodium hydroxide (NaOH) Himedia Cat# TC460M 4% Sodium hypochlorite (NaClO) Corclear N/A Dimethyl sulfoxide (DMSO) Sigma Cat# D5879 Absolute ethanol Jebsen & Jessen (GmbH & Co.) Cat# 58051 LO5 Methanol Merck Cat# 67-56-1 Experimental models: Organisms/strains Arabidopsis thaliana: Columbia; pPIN1::gPIN1:GFP (7-dpg) Gordon et al. 3 ; Kareem et al. 4 N/A Arabidopsis thaliana : Columbia; pXTH9::gXTH9:YFP, pPIN1::gPIN1:GFP (7-dpg) Varapparambath et al. 1 N/A Software and algorithms Fiji (ImageJ) Schneider et al. 6 https://imagej.net/software/fiji/ ZEN image processing software Zeiss https://www.micro-shop.zeiss.com/en/de/softwarefinder/software-categories/zen-black/zen-black-system/ Adobe Photoshop CS6 Adobe Acrobat N/A Adobe Illustrator Adobe Acrobat N/A Excel Microsoft N/A Other Arabidopsis growth chamber Percival AR-100L3 Laminar air flow Microfilt N/A Refrigerator Whirlpool FF2-550 WLP 1G2211 Sterile disposable square Petri dish (120 mm × 120 mm) Himedia PW050 Sterile disposable round Petri dish (35 mm) Tarsons 460035 Micro centrifuge Eppendorf Centrifuge 5420 Micro centrifuge tubes (1.5 mL) Tarsons 500010 PhytaWrap (2 inch) Himedia PLA002 10 mL syringes with needle Dispovan 13484 Syringe driven filter (45 μm pore size) Whatman Uniflo 9914-2504 Vanna’s micro-scissors (straight) Vanna’s AGT5223 Forceps Dumont Style 35A Round glass Petri dish (150 mm × 25 mm) Riviera 23755 52 Zeiss LSM 880 Zeiss N/A ZEN2.3 SP1 black edition Zeiss N/A Universal mounting frame A-K (D) - frame size 160 × 116 mm Zeiss 410800-1000-000 40× Water-dipping Objective ( W “ Plan - Apochromat ” 40×/1.0 DIC M27) Zeiss 421462-9900 Open in a separate window .

Techniques: Recombinant, Cell Culture, Software